| 猪瘟兔化弱毒E2基因重组杆状病毒的构建及抗体制备 |
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| 引用本文： | 范学政. 猪瘟兔化弱毒E2基因重组杆状病毒的构建及抗体制备[J]. 中国兽药杂志, 2015, 49(1): 6-9 |
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| 作者姓名： | 范学政 |
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| 作者单位： | 中国兽医药品监察所 |
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| 摘 要： | 为制备猪瘟特异性抗体,将猪瘟兔化弱毒E2基因序列修饰后克隆到pFastBac1质粒载体,经转座、转染后构建重组杆状病毒,并用纯化的重组猪瘟E2蛋白免疫大耳白兔制备特异性抗体。实验结果表明成功获得能分泌重组猪瘟E2蛋白的杆状病毒,使用纯化的重组猪瘟E2蛋白制备的猪瘟抗体具有良好的特异性和敏感性,为猪瘟病毒荧光抗体染色液的制备奠定了基础。
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| 关 键 词： | 猪瘟病毒 E2基因 杆状病毒表达,抗体制备 |
| 收稿时间： | 2014-10-09 |
| 修稿时间： | 2014-12-11 |
Construction of Recombinant Baculovirus Expressing HCLV E2 Gene and Antibody Preparation |
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| Affiliation: | China Institute of Veterinary Drug Control |
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| Abstract: | In order to prepare antibody for detection of classical swine fever virus(CSFV), the E2 gene of vaccine strain hog cholera lapinised virus(HCLV) and Mels, 6His sequence were cloned into vector pFastBac1, through thransposon and transfection, a recombinant baculovirus expressing HCLV E2 Gene was constructed. E2 protein was purified and immunized in rabbits, serum was purified through Protein A, confirmed by indirect FA, the antibody against CSFV was prepared. |
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| Keywords: | CSFV, E2 gene, BEVS, Antibody Preparation |
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