葡萄感染霜霉病菌后几种信号物质的变化

以3个抗病性不同的酿酒葡萄品种(‘西拉’‘霞多丽’‘赤霞珠’)叶片为材料,检测霜霉病菌侵染后几种病原相关信号物质,如脱落酸(ABA)、过氧化氢(H2O2)、一氧化氮(NO)、茉莉酸(JA)和乙烯(ETH)等的变化,以探讨葡萄抵御霜霉病菌的信号转导机制。结果表明,H2O2和NO是葡萄感应霜霉病菌的早期信号分子,且抗性较强的品种‘西拉’的变化最为明显;JA和ABA是抗性较差品种‘赤霞珠’应答霜霉病的信号组分。而3个抗性不同葡萄品种的乙烯变化无明显差别。推测H2O2、NO、JA和ABA均参与了葡萄对霜霉病菌的防御反应,但不同品种间信号转导组分可能存在差异。

Variation of signal substances in grape cultivars in response to Plasmopara viticola

Several disease-resistant signal substances, such as abscisic acid (ABA), hydrogen peroxide (H_2O_2), nitric oxide (NO), jasmonie acid (JA) and ethylene (ETH), in the leaves of 3 grape cultivars, 'Syrah', 'Char-donnay' and 'Cabernet Sauvignon', with different resistance to Plasmopara viticola, were studied in order to detect the signal transduction mechanisms. The results showed that H_2O_2 and NO were involved in the disease response, and 'Syrah' showed strong resistance! JA and ABA were involved in the disease response of the suscep-tible variety 'Cabernet Sauvignon'. Moreover, the level of ETH had no signifieant difference among the 3 culti-vars used. It was presumed that H_2O_2, NO, JA and ABA were involved in resistance against the downy mildew disease, but there were significantly different reactions among the three cultivars employed.