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Screen of Bovine Mammary Gland Epithelial Cell Specifcity Promotor
Authors:Liu Xiao-fei  Li Qing-zhang  Qiu You-wen  Gao Xue-jun
Institution:1. Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
2. Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
Abstract:Three lactoproteins (α-S1-casein, β-lactoglobulin, and β-casein) promotors were cloned, sequenced and compared relative luciferase expression. The results showed that the promotor activity of bovine α-S1-casein gene was the best, and would be used to produce pharmaceutically and medically important proteins in the mammary gland of transgenic animals and also for the construction of an inducible eukaryotic expression vector.
Keywords:lactoprotein  screen  specificity  promotor
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