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Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites |
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| Authors: | Anahit Penesyan Francesco Ballestriero Malak Daim Staffan Kjelleberg Torsten Thomas Suhelen Egan |
| Institution: | 1.School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio-Innovation, University of New South Wales, Sydney 2052, New South Wales, Australia; E-Mails: (A.P.); (F.B.); (M.D.); (S.K.); (T.T.);2.Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney 2109, New South Wales, Australia ;3.The Singapore Center on Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore |
| Abstract: | A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the “hit-rate” and unmasking multiple activities from the same bacterial source. |
| Keywords: | antibacterial antinematode functional genomic screen fosmid libraries marine bacteria marine bioactives Caenorhabditis elegans |