脂质体-禽巴氏杆菌荚膜抗原的免疫研究

将禽巴氏杆菌荚膜粗提物经SephadexG—200分子筛凝胶过滤层析,获得纯化荚膜抗原;然后用反相蒸发法制备脂质体-荚膜抗原(A组),并以单纯荚膜抗原(B组)、禽霍乱“731”菌苗(C组)作对照,进行免疫试验,以酶标单克隆抗体间接ELISA,分别检测IgM和IgG的效价.结果,免疫后3个组的IgM效价相差都不显著(P>0.05),A组的IgG效价明显增高,与其他两组差异非常显著(P<0.01).3个组的IgM、IgG消长规律一致,IgM于免疫后7d开始上升,峰值在14d,其后下降迅速,第8周后降至免疫前水平,IgG于免疫后14d开始明显上升,峰值在28d,其后缓慢下降.保护率,A、B、C组分别为67％、33％、33％.结果表明,脂质体对禽巴氏杆菌荚膜抗原具有明显的佐剂作用.

Immunological Studies on Liposome -enclosed Pasteurellar Capsule Antigen

The capsule crude extracts of Pasteurella multocida of avian origin were purifiedby Sephadex G - 200 gel filtration chromatography. Liposome-capsule antigen prepared by re-verse phase evaporation method (REM) was used as the immunizing antigen in group A, capsuleantigen and "731" vaccine were used in the control groups (B,C). After immunization of chick-ens with these antigens, the responses of specific IgM and IgG were detected with the indirectELISA. The titers of IgM were not different significantly among the immunized chickens in 3groups (P>0.05), but there was apparent difference between the IgG titers of test group andthose of control groups (P<0.01). The fluctuation patterns of IgM and IgG responses in 3groups were alike. IgM titers started to rise on 7th day after immunization, their peak value oc-curred on 14th day and declined rapidly later on, and reached the normal levels till 8th week.IgG began to rise on 14th day and reached the peak on 28th day, then declined slowly. The pro- tective rates of the groups A,B,C were 67%, 33% and 33%, respectively. It suggested that theliposome has an immunological adjuvant, effect on capsule antigen of Pasteurella multocida of a-vian origin.