葡萄不同器官直接再生不定芽研究

以葡萄继代试管苗的叶片﹑叶柄、茎段和根为试材,进行器官直接再生体系建立的研究。结果表明:MS是诱导葡萄器官直接再生较好的基本培养基,葡萄叶片是最佳的诱导材料;不定芽的诱导以MS+BA(1.5～2.0mg/L)+IBA(0.01mg/L)的激素组合最好,“红地球”、“维多利亚”、“甘农18号”的叶片均直接产生了较高的不定芽;在BA1.5～2.0mg/L最适范围,生长素浓度过高不利于器官直接产生不定芽。

Study on direct regenerating adventitious buds from different organs of grape

Direct organs regeneration system was studied using the leaves, petioles, stems and the roots of test-tube plantlets of grape .The results indicated that the best medium for regeneration were MS medium and the best explant was leaf. The optimum medium for inducing adventitious buds from leaves as MS + 6-BA (1.5～2.0 mg/L) + IBA(0.01～0.1 mg/L), and the leaves of the cultivars such as Red Globe, Victoria and GN-18 produced higher adventitious buds. Within the concentration scope of BA from 1.5 to 2.0 mg/L, higher α-naphthalene acetic was unfavourble to produce directly adventitious bud from organs.