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转番茄铁载体基因(LeIRT2)八棱海棠对缺铁胁迫的响应
引用本文:渠慎春,张君毅,陶建敏,乔玉山,章镇.转番茄铁载体基因(LeIRT2)八棱海棠对缺铁胁迫的响应[J].中国农业科学,2005,38(5):1024-1028.
作者姓名:渠慎春  张君毅  陶建敏  乔玉山  章镇
作者单位:南京农业大学园艺学院,南京,210095
基金项目:国家自然科学基金资助项目(30370987)和江苏省应用基础研究资助项目(BJ200018)
摘    要: 在低铁(铁浓度为10-6mol·L-1)水培条件下,2个转番茄铁载体蛋白基因八棱海棠(Malus robusta Rehd.)株系表现出较强的抗缺铁胁迫能力。叶片叶绿素含量和净光合速率显著高于对照;叶片细胞间隙CO2浓度显著低于对照;根系的质子分泌和三价铁螯合物还原酶(FCR)活性均升高,明显强于同样条件下的非转基因植株。根系扫描电镜结果显示,转基因株系根系的吸收区和根毛的表面结构皱折增多,表面积增大。

关 键 词:八棱海棠  LeIRT2  缺铁胁迫
收稿时间:2004-6-24

Physiological Reaction on Resistance to Iron Deficiency in Transgenic Lines of Malus robusta by Introducing LeIRT2 Gene
QU Shen-chun,ZHANG Jun-yi,Tao Jian-min,QIAO Yu-shan,ZHANG Zhen.Physiological Reaction on Resistance to Iron Deficiency in Transgenic Lines of Malus robusta by Introducing LeIRT2 Gene[J].Scientia Agricultura Sinica,2005,38(5):1024-1028.
Authors:QU Shen-chun  ZHANG Jun-yi  Tao Jian-min  QIAO Yu-shan  ZHANG Zhen
Abstract:Two transgenic plants of Malus robusta were hydroponically planted to test their efficiency to iron-deficiency(iron concentration 10-6mol·L-1), and the tolerance was found in these transgenic plants. Their leaf chlorophyll content and net photosynthetic rate were much higher than those of control, which showed severe chlorosis under the same condition. The rate of root H+ excretion and ferric-chelate reductase activity increased in transgenic lines and substantially higher than those of control. Scanning photos of root absorption region and root hair by electronic microscope in transgenic lines and non-transgenic plants were different under same iron treatment.
Keywords:Malus robusta  LeIRT2  Tolerance to iron-deficiency
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