| 木霉菌对香蕉枯萎病菌的抑制作用 |
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| 引用本文： | 钟小燕,梁妙芬,甄锡壮,赖进为,赖锡隆.木霉菌对香蕉枯萎病菌的抑制作用[J].果树学报,2009,26(2). |
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| 作者姓名： | 钟小燕 梁妙芬 甄锡壮 赖进为 赖锡隆 |
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| 作者单位： | 钟小燕,梁妙芬,甄锡壮,ZHONG Xiao-yan,LIANG Miao-fen,ZHEN Xi-zhuang(开平柏丽宝生物化学有限公司,广东开平,529300);赖进为,赖锡隆,LAI Jin-wei,LAI Xi-long(开平柏丽宝生物化学有限公司,广东开平,529300;三惠生物科技有限公司,香港九龙,999077)
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| 摘 要： | 实验室分离到1株对香蕉枯萎病菌(Fusarium oxysporum f.sp.cubens)有明显抑制作用的菌种,经18SrDNA鉴定为木霉属,命名为Trichoderma sp.G2。采用固体和液体培养法对菌株G2抑制香蕉枯萎病菌的生长进行了初步研究。结果表明,在对峙试验的平板上,7d香蕉枯萎病菌完全被G2的短绒状菌丝及分生孢子梗丛覆盖;在拮抗平板上,G2发酵液的平均抑制率约为90.4%,挥发性物质平均抑制率达68.3%,可见挥发性物质对抑制病原菌的生长起了重要的作用。在浓度为1.0×107cfu·mL-1香蕉枯萎病菌液体培养中,按体积比为20%接种G2,10d后镜检发现病原菌的菌丝及孢子大部分出现融解,表明菌株G2对香蕉枯萎病菌有明显的拮抗作用。
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| 关 键 词： | 香蕉 木霉菌 香蕉枯萎病菌 抑制作用 |
Study on the inhibition of Trichoderma sp.against Fusarium oxysporum f.sp.cubense in banana |
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| ZHONG Xiao-yan,LIANG Miao-fen,ZHEN Xi-zhuang,LAI Jin-wei,LAI Xi-long.Study on the inhibition of Trichoderma sp.against Fusarium oxysporum f.sp.cubense in banana[J].Journal of Fruit Science,2009,26(2). |
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| Authors: | ZHONG Xiao-yan LIANG Miao-fen ZHEN Xi-zhuang LAI Jin-wei LAI Xi-long |
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| Institution: | 1Kaiping PICO Bio-Technology CO.Ltd;Kaiping;Guangdong 529300 China;2Trinity Bio-Technology CO.Ltd;Kowloon;Hong Kong 999077 China |
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| Abstract: | A fungal strain,designated as G2,with remarkable ability of effective antagonist against Fusarium oxysporum f.sp.cubens,was isolated and identified as Trichoderma sp.based upon 18S rDNA gene analysis.With solid and liquid cul-tures,the inhibitive efficacy to the growth of Fusarium oxysporum f.sp.cubens was primarily studied.The experimental re-sults showed that the cells of Fusarium oxysporum f.sp.cubens were completely covered by short fiber-like hyphace and spore stem of G2 within 7 days in the dual cultu... |
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| Keywords: | Banana Trichoderma sp Fusarium oxysporum f sp cubens Antagonism |
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