栽培稻与普通野生稻BC_1群体分子连锁图的构建和株高的QTL分析

本研究用江西东乡普野和桂朝 2号的 115株 BC1群体 ,构建了一个长度为 1418.2 c M、包含 12 0个RFL P标记的遗传图谱 ,标记间的平均距离为 11.8c M。该图谱除第 1染色体短臂上的标记的顺序与日本水稻基因组计划发表的图谱不同外 ,其他染色体上相对应的标记的顺序及标记之间的遗传距离基本一致。该图谱为定位栽、野之间重要的分类性状和农艺性状以及进一步研究野生稻进化到栽培稻的分子进化机理奠定了基础。利用该图谱 ,对控制株高的 QTL s分析结果表明 ,控制株高有 6个 QTL s,他们分别位于第 1,3,4 ,5 ,8和 9染色体上 ,其中位于第 1染色体 C95 5— R1613间为 1个主效基因 ,并对主效基因的来源进行了讨论。最后作者提出 ,在野生稻驯化为栽培稻的过程中 ,株高由高变矮是微效基因突变与主效基因突变相结合并通过长期积累而成的

Genetic Linkage Map Constructed by Using a BC_1 Population and QTL Analysis of Plant Height for Common Wild Rice and Cultivated Rice

A genetic map including 120 RFLP markers covering 1418.2cM has been constructed using a BC 1 population derived from a cross between common wild rice ( Oryza rufipogon Griff.) from Dongxiang, Jiangxi Province, China, and an elite indica variety "Guichao 2". There is 11.8cM between RFLP markers. The linkage orders and genetic distances of markers in this map are basically consistent with the map constructed by Rice Genome Program of Japan except the markers of short arm of Chromosome 1. The genetic map will be useful for study of the classification traits and agronomic characters between cultivated and wild rice, as well as molecular evolution mechanism of cultivated rice. The QTLs analysis of plant height indicated that there are six QTLs located at the region of C955- R1613 of Chromosome 1, G51-C1677 of Chromosome 3, G237-G331 of Chromosome 4, R3166-R1838 of Chromosome 5, R2976-C1115 of Chromosome 8 and R1687-C1454 of Chromosome 9 respectively. The detected QTLs affecting this key agronomic trait for wild rice and cultivated rice will be useful for study of molecular evolution mechanism of cultivated rice and improvement of plant height.