球茎甘蓝小孢子培养中影响胚诱导的几个因素

以10份球茎甘蓝品种(系)为试材,采用游离小孢子培养方法,研究影响胚状体发生的因素。结果表明:基因型是影响胚状体发生的关键因素,不同基因型间胚诱导率存在显著差异;初花期为最理想的取蕾时期;添加适量激素能够提高胚诱导率,6-BA的适宜浓度为0.2mg·L-1,NAA的作用因基因型而异;4℃低温预处理24h可以明显提高胚诱导率;添加0.25mg·mL-1活性炭不仅可以显著提高胚诱导率,而且能够改善胚状体发育状态。

Several Factors Affecting Embryoid Induction from Isolated Microspore in Kohlrabi ( Brassica oleracea L. var. caulorapa DC.)

Isolated microspore culture was carried out by ten varieties（strains）of kohlrabi（Brassica oleracea L. var. caulorapa DC.）. It was acknowledged that the donor plant genotype is the key factor affecting microspore culture in kohlrabi. There were significant differences existing in embryoid inductions with different genotypes. Initial flowing stage was the optimum drawing time. Adding suitable quantity of hormone could raise embryoid induction rate. The suitable concentration of 6-BA was 0.2 mg·L^-1. The effects of NAA were different according to different genotypes. Pretreatment of 4 ℃ low-temperature for 24 h could increase the frequency of embryoid induction and adding 0.25 mg·mL^-1 active carbon could significantly promote embryoid induction and improve embryoid development.