| 陇东野生紫花苜蓿过氧化物酶同工酶分析 |
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| 引用本文： | 陈立强,师尚礼,王彩芬.陇东野生紫花苜蓿过氧化物酶同工酶分析[J].草地学报,2009,17(6):772-778. |
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| 作者姓名： | 陈立强 师尚礼 王彩芬 |
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| 作者单位： | 甘肃农业大学草业学院,草业生态系统教育部重点实验室,中美草地畜牧业可持续发展研究中心,兰州,730070 |
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| 基金项目： | "十一五"国家科技支撑计划，，，农业部公益性行业(农业)科研专项 |
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| 摘 要： | 利用聚丙烯酰胺垂直板凝胶电泳技术对陇东野生紫花苜蓿(Medicago sativa L. cv. Longdong)及其他38个紫花苜蓿品种的过氧化物酶(POD)同工酶酶谱特征进行分析,通过对供试材料间欧氏距离的计算和聚类分析,初步探讨了39个紫花苜蓿材料间的亲缘关系.结果表明:供试材料共表现出11条酶带,且都具有4条主酶带,其中陇东野生紫花苜蓿表现出7条酶带;用组间均连法进行聚类分析,39个供试材料可聚为6类,其中陇东野生紫花苜蓿单独为一类,且与其他38个供试材料间的欧氏距离介于0.748～1.622,平均值(1.088)远远高于其他供试材料间的平均欧氏距离(0.533～1.029),表明它们之间的遗传距离相对较大.由此可确定陇东野生紫花苜蓿与其他供试材料间的亲缘关系较远,为一相对独立的紫花苜蓿种质资源,可通过育种途径扩大栽培品种的遗传基础.
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| 关 键 词： | 陇东野生紫花苜蓿 过氧化物酶同工酶 亲缘关系 紫花苜蓿种质 |
Analysis of Longdong Wild Alfalfa Based on Peroxidase Isozymes Zymogram |
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| CHEN Li-qiang,SHI Shang-li,WANG Cai-fen.Analysis of Longdong Wild Alfalfa Based on Peroxidase Isozymes Zymogram[J].Acta Agrestia Sinica,2009,17(6):772-778. |
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| Authors: | CHEN Li-qiang SHI Shang-li WANG Cai-fen |
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| Institution: | CHEN Li-qiang,SHI Shang-li*,WANG Cai-fen(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassl, Ecosystem,Ministry of Education Sino-U.S.Centers for Grazingl, Ecosystem Sustainability.Lanzhou 730070,China) |
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| Abstract: | Longdong wild alfalfa(Medicago sativa L.cv.Longdong) is a rhizomatous perennial autotetraploid(2n=4x=32) legume forage found in the Longdong region of Gansu.It is considered to be Medicago sativa on the basis of its morphology,and has potential as forage because of its high branching ability,presence of rhizomes and its tolerance to trampling,cold and drought.but little is known about the genetic differences between Longdong wild alfalfa and cultivars.Peroxidase(POD) isozymes of Longdong wild alfalfa and ot... |
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| Keywords: | Longdong wild alfalfa Peroxidase isozymes Genetic relationship Alfalfa germplasm |
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