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| 鸡卵清蛋白基因调控序列指导人促红细胞生成素转基因鸡的制备 | |
| 引用本文： | 杜立新,赵英会,张念华.鸡卵清蛋白基因调控序列指导人促红细胞生成素转基因鸡的制备[J].山东农业大学学报(自然科学版),2004,35(3):321-324. |
| 作者姓名： | 杜立新 赵英会 张念华 |
| 作者单位： | 1. 中国农业科学院畜牧研究所,北京,100094;山东农业大学动物科技学院,山东,泰安,271018 2. 山东农业大学动物科技学院,山东,泰安,271018;泰山医学院,山东,泰安,271000 3. 泰山医学院,山东,泰安,271000 |
| 基金项目： | 山东自然科学基金资助项目 (Z990D0 3) |
| 摘 要： | 本研究将鸡卵清蛋白5端调控区调控人促红细胞生成素的pOV-hEPO特异表达载体用脂质体包埋，和供体细胞融合后，显微注射入450枚受体胚盘，采用代用蛋壳法培养制备转基因鸡，PCR和斑点杂交检测外源基因已整合入早期鸡胚胎生殖系统，为制备表达人促红细胞生成素的鸡输卵管生物反应器奠定了基础。 |
| 关 键 词： | 促红细胞生成素 卵清蛋白 基因调控 EPO 脂质体 受体 早期 转基因鸡 代用蛋壳 调控区 |
| 文章编号： | 1000-2324(2004)03-0321-04 |
| 修稿时间： | 2003年12月31 |
THE PRODUCTION OF TRANSGENIC CHICKEN OF HEPO DIRECTED BY THE CHICKEN OVALBUMIN REGULATORY ELEMENTS |
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| DU Li-xin ,ZHAO Ying-hui ,ZHANG Niang-hua.THE PRODUCTION OF TRANSGENIC CHICKEN OF HEPO DIRECTED BY THE CHICKEN OVALBUMIN REGULATORY ELEMENTS[J].Journal of Shandong Agricultural University,2004,35(3):321-324. | |
| Authors: | DU Li-xin ZHAO Ying-hui ZHANG Niang-hua |
| Institution: | DU Li-xin 1,2,ZHAO Ying-hui 1,3,ZHANG Niang-hua 3 |
| Abstract: | The expression vector pOV-hEPO of human erythropoietin directed by the chicken ovalbumin regulatory elements was embedded in liposome. The donor cells suspended from stage X blastoderm and plasmid pOV-hEPO were co-cultured. Then the co-culture was microinjected into recipient blastoderm at the same stage of development. The recipient embryos were cultured with surrogate eggshell. PCR and Dot blot analysis indicated than hEPO gene are integrated into the genital gland in early embryos. The results irdicate that it is possible for the production of chicken ovalbumin bioreactor using Lipofection-mediated exogenous gene directed by the chicken ovalbumin regulatory elements. |
| Keywords: | human erythropoietin transgenic chicken oviduct bioreactor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |