Population structure of poplar rust Melampsora larici-populina in the UK inferred from AFLP |
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Authors: | M H Pei C Bayon C Ruiz I Tubby |
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Institution: | Rothamsted Research, Harpenden, AL5 2JQ;and;Forest Research, Alice Holt Lodge, Wrecclesham, Farnham, GU10 4LH, UK |
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Abstract: | AFLP profiles were generated from poplar leaf rust ( Melampsora larici-populina ) collected from Populus trichocarpa cv. Trichobel at six sites in the UK in 2001. Of a total of 145 isolates, 121 were unique AFLP phenotypes. On one occasion, the same AFLP phenotype was found at two sites. The analysis indicated that 81·88% of the total variation was attributable to differences among individuals within replicate plots, 3·47% to differences among plots within sites and 14·65% to differences among sites. Weir & Cockerham's θ was calculated as 0·138 and θ B , the Bayesian analogue of F ST, as 0·146. Overall, there was sufficient gene flow among different sites to prevent genetic drift, as Nm was calculated as 1·562 using θ and as 1·462 using θ B . Nei's genetic distance and population-assignment test indicated that the rust from a site in north-east England was differentiated from those from other sites. There was no significant correlation between genetic and geographical distances. The results indicate that sexual reproduction has a major influence on the relative lack of population structure of M. larici-populina in the UK. |
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Keywords: | analysis of molecular variance F ST gene flow Populus trichocarpa sexual reproduction |
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