沙丁胺醇残留的酶联免疫检测方法的建立

用自主制备的沙丁胺醇特异性抗体建立了针对沙丁胺醇残留的间接竞争酶联免疫检测方法,对该检测体系的灵敏度、准确度、精密度、特异性等性能进行了测定,并与高效液相色谱方法进行了对比性分析。结果显示:沙丁胺醇药物残留的间接竞争酶联免疫检测体系的检测范围为1～80ng/mL,灵敏度为0.58ng/mL,检测限为1ng/mL,回收率为70～99%,与盐酸克仑特罗、硫酸特布他林交叉反应率分别为107%、10%,与盐酸莱克多巴胺及肾上腺素的交叉反应率小于0.01%;采用HPLC方法进行沙丁胺醇药物残留的检测时,其检测范围为10μg/mL～200μg/mL,检测限为1μg/mL,回收率为80～95%。该方法与高效液相色谱法相比灵敏度较高,特异性强,检测结果准确度相近,但是在检测结果稳定性方面逊与HPLC方法。

Development of ELISA Method for Residue of Salbutamol

An indirect competitive ELISA system to detect the residue of salbutamol was developed with sabutamol antibody and the performance about the detection system such as the sensitivity ,the detection limit ,the recoveries ,the precision and the specification were tested. And as the control method to this detection system,one High performance liquid chromatographic method (HPLC) to the residue of salbutamol was developed. The result showed the calibration curve of this ELISA detection system had the linear detection of 1～80ng/mL,the sensitivity of 0.58ng/mL and the detection limit of 1ng/mL. The recoveries of salbutamol in pork were 70～99%,and this ELISA detection system had respectively 107% cross-reactivity to clenbutarol and 10% to terbutalin,however,it had little cross-reactivity below 0.01% to Ractopamine and adrenaline. As the control detection systems,HPLC method had the linear detection of 10～200ug/mL,the sensitivity of 1ug/ml. The recoveries of salbutamol in pork were 80～95% through HPLC. In conclusion,compared with HPLC method,ELISA method had better sensitivity,specificity and approximative recoveries,but as estimated,ELISA method was not perfect enough in detec-tion result stability as HPLC.