| 促肝细胞再生磷酸酶-3蛋白在5株癌细胞中的表达 |
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| 引用本文： | 何太平,谢富华,侯庆华,莫丽儿,梁念慈. 促肝细胞再生磷酸酶-3蛋白在5株癌细胞中的表达[J]. 湛江医学院学报, 2007, 25(1): 1-3 |
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| 作者姓名： | 何太平 谢富华 侯庆华 莫丽儿 梁念慈 |
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| 作者单位： | 广东医学院;广东医学院;广东医学院 |
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| 基金项目： | 广东省中医药管理局资助项目 , 广东省湛江市科技攻关项目 , 广东医学院校科研和教改项目 |
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| 摘 要： | 目的研究促肝细胞再生磷酸酶-3(phosphatase of regenerating liver-3,PRL-3)在5株癌细胞(HO-8910PM、CNE-2Z、A549、JAR和BEL-7402)中的表达情况,为选择合适的细胞株克隆PRL-3基因奠定基础。方法用Western blot分析PRL-3在5株癌细胞(HO-8910PM、CNE-2Z、A549、JAR和BEL-7402)中的表达。结果PRL-3蛋白在5株癌细胞中都有表达:在HO-8910PM与CNE-2Z中呈高度表达,在A549和BEL-7402中呈中等程度表达,而在JAR中呈低度表达。结论PRL-3在5株癌细胞中均有表达,可选择高表达的HO-8910PM或CNE-2Z细胞克隆PRL-3基因。
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| 关 键 词： | 肿瘤 转移 表达 PRL-3 |
| 文章编号： | 1005-4057(2007)01-0001-03 |
| 修稿时间： | 2006-10-09 |
The expression of phosphatase of regenerating liver-3 in five carcinoma cell lines |
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| HE Tai-ping,XIE Fu-hua,HOU Qing-hua,MO Li-er,LIANG Nian-ci. The expression of phosphatase of regenerating liver-3 in five carcinoma cell lines[J]. Journal of Guangdong Medical College, 2007, 25(1): 1-3 |
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| Authors: | HE Tai-ping XIE Fu-hua HOU Qing-hua MO Li-er LIANG Nian-ci |
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| Affiliation: | 1. Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, P. R. China |
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| Abstract: | Objective To observe the expression of phosphatase of regenerating liver-3(PRL-3) in five cancer cell lines HO-8910PM,CNE-2Z,A549,JAR and BEL-7402,on which the appropriate cell lines will be chosen to clone PRL-3 gene.Methods Western blot analysis was used to detect the expression of PRL-3.Results The expression of PRL-3 was clearly detected in all the five carcinoma cell lines, with the highest expression in HO-8910PM and CNE-2Z,moderate expression in A549 and BEL-7402,and the lowest expression in JAR.Conclusion The expression of PRL-3 was high in cell lines HO-8910PM and CNE-2Z,which might be suitable to server as the cell line for cloning PRL-3 gene. |
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| Keywords: | cell line HO-g910PM CNE-2Z A549 JAR BEL-7402 PRL-3 |
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