| 辣椒叶片总RNA提取与质量分析 |
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| 引用本文： | 杨继涛,张素勤,耿广东,苏丹.辣椒叶片总RNA提取与质量分析[J].西北农业学报,2009,18(6):217-220. |
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| 作者姓名： | 杨继涛 张素勤 耿广东 苏丹 |
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| 作者单位： | 杨继涛(西北农林科技大学,陕西杨凌,712100);张素勤,耿广东,苏丹(贵州大学,农学院,贵州贵阳,550025) |
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| 基金项目： | 贵州省科学技术基金(黔科合J字(2006)2052号、，贵州大学引进人才基金，205，国家科技支撑计划项目 |
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| 摘 要： | 用3种不同方法提取辣椒叶片的总RNA,研究辣椒叶龄和基因型对总RNA提取的影响和筛选辣椒总RNA提取的最佳方法.结果表明,方法2为辣椒叶片总RNA提取的最佳方法;从新叶中提取的总RNA的亮度高,完整性最好,中龄叶次之,老叶最差;辣椒材料0860-1总RNA的28 S和18 S条带明亮,质量浓度最高,材料0876和08122的这些条带较暗,质量浓度低.
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| 关 键 词： | 辣椒 叶片 RNA提取 质量 |
| 收稿时间： | 2009/6/24 0:00:00 |
| 修稿时间： | 2009/9/2 0:00:00 |
Extraction and Quality Analysis of Total RNA in Leaves of Hot Pepper |
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| YANG Jitao,ZHANG Suqin,GENG Guangdong and SU Dan.Extraction and Quality Analysis of Total RNA in Leaves of Hot Pepper[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(6):217-220. |
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| Authors: | YANG Jitao ZHANG Suqin GENG Guangdong and SU Dan |
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| Institution: | College of Horticulture, Northwest A&F University, Yangling Shaanxi 712100, China;Agricultural College, Guizhou University, Guiyang Guizhou 550025, China;Agricultural College, Guizhou University, Guiyang Guizhou 550025, China;Agricultural College, Guizhou University, Guiyang Guizhou 550025, China |
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| Abstract: | Three methods were applied to extract total RNA from the leaves in order to understand the effects of leaf ages and genotypes and choose the best method for total RNA extraction in hot pepper in the present study. The results showed that the 28 S and 18 S bands extracted by method 2 were strong, and the quality and concentration of the total RNA were high. The lightness and quality of the total RNA from young leaves extracted by method 2 were the highest, next came medium leaves, and then old ones. The 28 S and 18 S bands from the leaves of line 0860-1 extracted by method 2 were bright, and total RNA concentration was the highest; but those of the lines 0876 and 08122 were dark, and the concentration was low. Therefore, the extraction effect of total RNA might be influenced by hot pepper genotypes. |
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| Keywords: | Hot-pepper Leaf RNA extraction Quality |
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