巴东红三叶胚性细胞悬浮系的建立

以红三叶(Trifolium pratense)野生品种巴东红三叶的叶片为外植体,在MB-1至MB-9的固体培养基上诱导愈伤组织,筛选出最佳培养基MB-9.愈伤组织形成以后,均经MB-9固体培养基上继代培养3～4次后,获得胚性愈伤组织,然后转至改良的MB-9液体培养基中,经强化培养两个月后,获得胚性细胞悬浮系.结果表明,缩短换液时间,每隔3～4d换液1次,可加快胚性细胞悬浮系的建立.

Establishment of Embryogenic Cell Suspension of Trifolium pratense cv Badong

The leaves of Trifolium pratense cv Badong were used as explants in this experiment.The calli were induced from the medium ranging from MB-1 to MB-9 in order to select the best medium,and MB-9 was found to be the best.The embryogenic calli were got after calli were subcultured three or four times in MB-9.Then the embryogenic calli converted into MB+3.0 mg/L 2.4-D+0.70 mg/L BA+0.2 mg/L NAA+2 mg/L glycine+500 mg/L CH+300 mg/L cane sugar for two months for getting embryogenie cell suspension.The results showed that shortening the interval of liquiding(liquid every 3～4 d)may speed up the establishment of embryogenic cell suspension