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Storage of broccoli pollen in liquid nitrogen
Authors:P. Crisp  B. W. W. Grout
Affiliation:(1) National Vegetable Research Station, CV 35 9EF Wellesbourne, Warwicks, UK;(2) North East London Polytechnic, Romford Road, E15 4LZ London, UK;(3) Present address: Plymouth Polytechnic, Drake Circus, PL4 8AA Plymouth, UK
Abstract:Summary Broccoli pollen which had been stored in liquid nitrogen retained its viability, but seed produced using this pollen rapidly lost its germinability. Plants raised from this seed, and their progenies, gave no indication of genetic damage resulting from the low temperature treatment of pollen.
Keywords:Brassica oleracea var. italica  broccoli  genetic conservation  cryopreservation  pollen
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