| 玉米(Zea mays L.)两个广谱抗病基因rip和pal 1的原位杂交定位 |
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| 引用本文： | 王玲,宋运淳,宁顺斌,李立家,刘立华. 玉米(Zea mays L.)两个广谱抗病基因rip和pal 1的原位杂交定位[J]. 作物学报, 1999, 25(5): 639-642 |
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| 作者姓名： | 王玲 宋运淳 宁顺斌 李立家 刘立华 |
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| 作者单位： | 武汉大学生命科学学院，湖北武汉，430072 |
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| 基金项目： | 国家自然科学基金，国家教委博士点基金资助项目. |
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| 摘 要： | 本文以玉米自交系黄早四为供试材料，采用cDNA为探针，对低拷贝小片段的rip和pall基因进行了定位。用生物素标记和相应的酶联级联放大检测系统，在第8和第3染色体长臂上检测到rip基因的杂交信号，信号与着丝粒百分距离分别为23.66±1.32和72.47±3.16。在第5和第4染色体长臂以及第2染色体短臂上检测到pal l基因位点，信号与着
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| 关 键 词： | 玉米 rip基因 pal l基因 原位杂交 |
| 收稿时间： | 1998-08-29 |
| 修稿时间： | 1998-12-18 |
Mapping of the Two Resistance Genes rip and pall by in situ Hybridiza-tion |
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| Wang Ling Song Yunchun Ning Shunbin Li Lijia Liu Lihua. Mapping of the Two Resistance Genes rip and pall by in situ Hybridiza-tion[J]. Acta Agronomica Sinica, 1999, 25(5): 639-642 |
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| Authors: | Wang Ling Song Yunchun Ning Shunbin Li Lijia Liu Lihua |
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| Affiliation: | School of Life Sciences, Wuhan University, Wuhan, 430072 |
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| Abstract: | In this paper，the low copy genes rip and pall were physically mapped by in situ bybridization. With biotinylated cDNA probes of the two genes and DAB detection，the signals of the rip gene were detected in the long arms of the eighth and third chromosomes. The percent distances from centromere to d |
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| Keywords: | Maize Gene rip Gene pal1 In situ hybridization |
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