| 西瓜杂交种遗传多态性的SRAP标记分析 |
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| 引用本文： | 李严,张春庆.西瓜杂交种遗传多态性的SRAP标记分析[J].园艺学报,2005,32(4):643-647. |
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| 作者姓名： | 李严 张春庆 |
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| 作者单位： | (山东农业大学农学院, 泰安271018) |
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| 摘 要： | 利用新型分子标记SRAP ( Sequence2Related Amp lified Polymorphism) 进行西瓜杂交种遗传多态性的研究, 利用25个引物组合对当前生产上推广的20个西瓜杂交种进行扩增, 从中筛选得到20个多态性引物组合, 共产生135个多态性条带, 平均每个引物组合产生71.1个多态性条带, 显示了较高的多态性比率。聚类分析20个引物组合的扩增结果, 20份材料分为3大类, Jaccardps相似系数在0.29～0.86之间。
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| 关 键 词： | 西瓜 杂交种 多态性 SRAP |
| 文章编号： | 0513-353X（2005）04-0643-05 |
| 收稿时间： | 2004-11-11 |
| 修稿时间： | 2004-11-112005-01-12 |
Studies on Genetic Diversity with a Molecular Marker SRAP of Watermelon Hybrids |
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| Li Yan,Zhang Chunqing.Studies on Genetic Diversity with a Molecular Marker SRAP of Watermelon Hybrids[J].Acta Horticulturae Sinica,2005,32(4):643-647. |
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| Authors: | Li Yan Zhang Chunqing |
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| Institution: | (College of Agronomy, Shandong Agricultural University, Tai'an 271018, China) |
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| Abstract: | Study on genetic diversity of watermelon could benefit the research of cultivar property right protecting,seed purity testing,and genetic breeding.A new molecular marker system-SRAP(Sequence-Related Amplified Polymorphism) was firstly applied on 20 watermelon hybrids genetic diversity research.20 primer pairs selected from 25 amplified 135 polymorphic bands with an average of 7.11 polymorphic bands per primer pair.Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 20 primer pairs showed 20 hybrids could be distinguished into three main groups.Jaccard's similarity coefficient ranged from 0.29-0.86. |
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| Keywords: | Watermelon Hybrid Polymorphism SRAP |
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