Haploid induction from F1 hybrids between CMS shallot with Allium galanthum cytoplasm and common onion by unpollinated flower culture |
| |
Authors: | Endang Sulistyaningsih Ken-ichiro Yamashita Yosuke Tashiro |
| |
Affiliation: | (1) Laboratory of Biotechnology and Plant Breeding, Faculty of Agriculture, Saga University, Saga 840-8502, Japan |
| |
Abstract: | The induction of haploid plants from F1 hybrids between CMS shallot with Allium galanthum cytoplasm and common onion was examined. Starting with 535 unpollinated flowers cultured in B5 medium 25 seedlings from part henogenetic embryos were obtained of which 13 seedlings survived. Eleven seedlings were determined as haploid plants (2n = x = 8) and 2 seedlings were doubled haploid plants (2n = 2x = 16). All haploid and doubled haploid plants preserved chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) from A. galanthum. Segregation in different characters was observed among the haploid plants. The haploid and doubled haploid plants exhibited the different combinations of genes from shallot and common onion. Crossing of the doubled haploid plants with other shallot strains, common onion cultivars or related species may produce excellent F1 hybrids for bulb production. This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | Allium cepa CMS haploid onion shallot unpollinated flower culture |
本文献已被 SpringerLink 等数据库收录! |