鹅掌楸属两种植物花粉品质和花粉管生长的研究

应用ＦＤＡ荧光染色法、离体和活体培养及看护培养等方法检验了鹅掌楸属两种木本植物花粉的品质，观察了开花期间花粉品质的变化，同时也观察统计了自然和人工授粉情况下，花粉在柱头上萌发及花粉管在花柱中生长的情况。研究结果表明：在离体条件下，柱头和子房对花粉的萌发和花粉管的生长有明显的促进作用；花粉在自己或异己的柱头和胚珠上萌发均良好，是亲和的，但花粉管通过花柱的比率较低，仅２４％。与本课题前期工作的研究结果相一致。并从数量调查和活体观察两个方面，再一次证实雄配子体的发育水平是影响结籽率的重要因子之一，花柱是不亲合性反应的发生区域。

TESTS OF POLLEN QUALITY AND DEVELOPMENT OF POLLEN TUBES IN LIRIDENDRON (MAGNOLIACEAE)

By the methods of fluorochroma(FDA),in vitro,in vivo and nursery stock growing,the pollen quality of the two woody plants, Liridendron chinense and L. tulipifera is examined,and the change in pollen quality was observed during the flowering period.Meanwhile,the pollen germination on the stigma and the growth of pollen tube in the style were investigated under the natural or artificial pollination condition.The results indicate that in vitro condition the pollen germination and pollen tube growth are promoted by the style and ovary tissue obviously.The pollens are affinity,and germinate well on oneself or alien stigma and ovule,however the rate of the pollen tube passing the style is low,only 24%,which is in accordance with our previous study.The results prove again that the developmental phase of the male gametophytes is one of the important factors which influence the seed setting rate,and the style is the region where the inaffinity reaction occurs.