Callus induction and plant regeneration from lateral shoots of herbaceous bamboo Mniochloa abersend |
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| Authors: | Qiaolu Zang Yulian Jiao Xiaomei Guo Fei Zhuge Kaiwun Yeh |
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| Affiliation: | 1. The Research Center for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an, China;2. Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei, Taiwan |
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| Abstract: | Callus induction and plant regeneration of Mniochloa abersend via lateral shoots were conducted in this study. Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg/L naphthaleneacetic acid (NAA) was effective for compact callus induction. Remarkably, calli on the MS medium with 0.1 mg/L 2,4-D yielded the highest folds of proliferation (8.01), and showed a high potential capacity to differentiate 1 year after subculture. In addition, the compact calli possessed 100% differentiation rate and generated more shoots that were green and strong in 1.0 mg/L kinetin and 1.0 mg/L NAA. Vigorous roots were generated in the 1/2MS supplemented with 0.5 mg/L indole-3-butyric acid, and the resultant plantlets exhibited 90% survival rate after they were hardened and transplanted. The established regeneration system of M. abersend provides a promising platform for bamboo gene function study. |
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| Keywords: | Callus culture lateral shoots Mniochloa abersend plant growth regulators plant regeneration |
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