大豆不同生育时期叶绿素含量QTL的定位及其与产量的关联分析

利用来自波高×南农94-156的151个RI家系检测与4个不同生育时期叶绿素含量（累积量、净增量）有关的QTL，并分析其与籽粒产量、表观生物学产量和表观收获指数的关系。结果表明，与叶绿素累积量有关的QTL位于D1a+Q、F、G、H、L和M连锁群上，每个QTL可解释表型变异的6.9%~23.4%。V6和R2期没有检测到2个年份均表达的QTL，而在R4期检测到4个在2个年份均表达的QTL（qccF.1、qccG.2、qccH.1和qccM.1），R6期仅检测到1个QTL（qccH.1）在2个年份均表达，该QTL在R4也表达。与叶绿素含量净增量有关的QTL位于B2和L连锁群上，在V6-R2时期没有检测到与叶绿素净增量有关的QTL，在B2和L连锁群上的两个QTL（qccB2-1.1和qccL.1）在R2-R4和R4-R6时期均表达，qccB2-1.1可解释表型变异的6.4%~9.8%，而qccL.1所解释表型变异达29.5%~31.3%。但这两个QTL在R2-R4和R4-R6时期表达的性质不同，且与2年均表达的籽粒产量QTL共位。这印证了生育后期叶绿素含量与籽粒产量间存在的极显著正相关。

QTL Mapping of Chlorophyll Content at Various Growing Stages and Its Relationship with Yield in Soybean [Glycine max (L.) Merr.]

Chlorophyll content of plant leaf is an important trait associated with photosynthesis,as a major photosynthetic pigment and index of crop field management and crop breeding.In the current study,a set of RIL population derived from Bogao and Nannong 94-156 was used to detect quantitative trait loci(QTLs) associated with chlorophyll content(CC) at four growing stages,and their correlations with three yield-related traits including seed yield,apparent biological yield and apparent harvest index were analysed.The primary results indicated that QTLs associated with the accumulation of chlorophyll content were detected and mapped on LG-D1a Q,F,G,H,L,and M,each QTL explained 6.9%-23.4% of phenotypic variation.There were no QTLs detected to be expressed across two years at the V6 and R2 growing stages.However,at the R4 stage four QTLs(qccF.1,qccG.1,qccH.1,and qccM.1) expressed in both years,and one of them(qccH.1) also expressed at R6 stage in two years.QTLs qccB2-1.1 and qccL.1 associated with the net increment of chlorophyll content were detected and mapped on LG-B2 and L,expressed from R2 to R4 and from R4 to R6,and explained 6.4%-9.8% and 29.5%-31.3% of phenotypic variation,respectively.qccB2-1.1 showed positive additive effect from R2 to R4 and negative additive effect from R4 to R6,while qccL.1 showed negative additive effect from R2 to R4 and positive additive effect from R4 to R6.These two QTLs co-located with QTLs for seed yield,confirming that there is significant correlation between seed yield and chlorophyll content at late growing stages.