紫秆海芋组织培养技术研究

用植物组织培养方法建立紫秆海芋快繁体系,结果表明:紫秆海芋外植体诱导成活最好的部位是芽,外植体诱导愈伤组织最佳培养配方是:MS+BA 1.5 mg/L+NAA 1.5 mg/L+碳粉3 g/L,诱导成活率高达100%,并且无污染;愈伤组织诱导成苗的最佳配方是:MS+TDZ 2.0 mg/L+NAA 1.0 mg/L,诱导成苗率为100%;继代增殖的最佳配方是:MS+BA 1.5 mg/L+KT 1.0 mg/L+NAA 0.5 mg/L+琼脂5.8 g/L,增殖率410%,并且植株长势良好;生根培养的最佳配方是:MS+BA 0.2 mg/L+NAA 1.0 mg/L+IBA 1.0 mg/L,并且平均根数、平均根长、平均根粗3个数值较理想。

Reserch on the Tissue Culture Technique ofAlocasia macrorrhiza

Rapid propagation system was established based on the culture techniquein vitro. Results were showed as follows. The explant which was most likely to survivein vitro was the bud. The callus were induced using the medium： MS＋BA 1.5 mg/L＋NAA 1.5 mg /L＋carbon powder 3 g/L, and the induction survival rate was up to 100% without contamination. The best formulations to induce the callus into seedlings were MS＋TDZ 2.0 mg/L＋NAA 1.0mg/L, and the seedling rate was 100%. The subculture micropropagation’s formula with 410% reproduction rate was MS＋BA 1.5 mg/L＋KT 1.0 mg/L＋NAA 0.5 mg/L＋ agar 5.8 g/L. The composition of rooting culture was MS＋BA 0.2 mg/L＋NAA 1.0 mg/L＋IBA 1.0 mg/L, obtaining ideal average root numbers, length, width.