不同基因型花生EMS诱变条件分析及突变系筛选 |
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引用本文: | 杨富军,王绍伦,高华援,刘海龙,周玉萍,李春洁,朱统国,孙晓苹,李晓伟. 不同基因型花生EMS诱变条件分析及突变系筛选[J]. 安徽农业科学, 2014, 0(33): 11778-11781 |
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作者姓名: | 杨富军 王绍伦 高华援 刘海龙 周玉萍 李春洁 朱统国 孙晓苹 李晓伟 |
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作者单位: | 吉林省农业科学院花生研究所,吉林公主岭,136100;吉林省公主岭市农机局,吉林公主岭,136100 |
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基金项目: | 国家花生产业技术体系项目,吉林省科技发展计划项目,吉林省自然科学基金项目 |
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摘 要: | 为明确不同基因型花生最佳甲基硫酸乙酯(EMS)诱变条件,以“四粒红”和“白沙1016”花生品种为材料,在0.30%、0.90% EMS溶液中浸泡3、5、7和9h,进行发芽试验.结果表明:以致死量达到50%为标准,多粒型花生的最佳处理条件是0.90% EMS溶液诱变7h,珍珠豆型花生则为0.90% EMS溶液诱变9h,多粒型花生对EMS响应明显.在M3、M4代突变系中,多粒型筛选出YD35、YD3、和YD223个高产株系,最高增产565 kg/hm2,珍珠豆型筛选出YZ4 、YZ2和YZ13个高产株系,最高增产570 kg/hm2.
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关 键 词: | 花生 多粒型 珍珠豆型 EMS诱变 突变系 |
Analyzing EMS Mutagenesis Conditions of Different Genotypes Peanut and Screening of Mutant Lines |
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Affiliation: | YANG Fu-jun,WANG Shao-lun,GAO Hua-yuan(Peanut Research Institute, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100) |
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Abstract: | In order to clarify the best EMS mutagenesis conditions of different peanut genotypes,with ‘ Silihong’ and‘ Baisha1016’ as materials,the germination experiments were conducted at 0.30%,0.90% EMS solution soaking for 3,5,7 and 9 h.Lethal to 50% of the standard,the results showed that the optimum treatment conditions of multi-grain type peanut is soaking into 0.90% EMS solution for 7 hours,and the vulgaris type peanut need soaking into 0.90% EMS solution for 9 hours.Multi-grain type peanut was significantly response for EMS.In the M3 and M4 generations mutant lines,multi-grain type peanut had selected three high-yield strains,YD35,YD3 and YD22,and the highest incremental was 565 kg/hm2 in yield.Vulgaris type peanut also had screened three high-yield strains,YZ4,YZ2 and YZ1,and the highest incremental was 570 kg/hm2 in yield. |
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Keywords: | Peanut Multi-grain type Vulgaris type EMS mutagenesis Mutant lines |
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