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不同基因型花生EMS诱变条件分析及突变系筛选
引用本文:杨富军,王绍伦,高华援,刘海龙,周玉萍,李春洁,朱统国,孙晓苹,李晓伟.不同基因型花生EMS诱变条件分析及突变系筛选[J].安徽农业科学,2014(33):11778-11781.
作者姓名:杨富军  王绍伦  高华援  刘海龙  周玉萍  李春洁  朱统国  孙晓苹  李晓伟
作者单位:吉林省农业科学院花生研究所,吉林公主岭,136100;吉林省公主岭市农机局,吉林公主岭,136100
基金项目:国家花生产业技术体系项目,吉林省科技发展计划项目,吉林省自然科学基金项目
摘    要:为明确不同基因型花生最佳甲基硫酸乙酯(EMS)诱变条件,以“四粒红”和“白沙1016”花生品种为材料,在0.30%、0.90% EMS溶液中浸泡3、5、7和9h,进行发芽试验.结果表明:以致死量达到50%为标准,多粒型花生的最佳处理条件是0.90% EMS溶液诱变7h,珍珠豆型花生则为0.90% EMS溶液诱变9h,多粒型花生对EMS响应明显.在M3、M4代突变系中,多粒型筛选出YD35、YD3、和YD223个高产株系,最高增产565 kg/hm2,珍珠豆型筛选出YZ4 、YZ2和YZ13个高产株系,最高增产570 kg/hm2.

关 键 词:花生  多粒型  珍珠豆型  EMS诱变  突变系

Analyzing EMS Mutagenesis Conditions of Different Genotypes Peanut and Screening of Mutant Lines
Institution:YANG Fu-jun,WANG Shao-lun,GAO Hua-yuan(Peanut Research Institute, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100)
Abstract:In order to clarify the best EMS mutagenesis conditions of different peanut genotypes,with ‘ Silihong’ and‘ Baisha1016’ as materials,the germination experiments were conducted at 0.30%,0.90% EMS solution soaking for 3,5,7 and 9 h.Lethal to 50% of the standard,the results showed that the optimum treatment conditions of multi-grain type peanut is soaking into 0.90% EMS solution for 7 hours,and the vulgaris type peanut need soaking into 0.90% EMS solution for 9 hours.Multi-grain type peanut was significantly response for EMS.In the M3 and M4 generations mutant lines,multi-grain type peanut had selected three high-yield strains,YD35,YD3 and YD22,and the highest incremental was 565 kg/hm2 in yield.Vulgaris type peanut also had screened three high-yield strains,YZ4,YZ2 and YZ1,and the highest incremental was 570 kg/hm2 in yield.
Keywords:Peanut  Multi-grain type  Vulgaris type  EMS mutagenesis  Mutant lines
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