| Rep-PCR技术及其应用现状 |
| |
| 引用本文： | 赵燕梅,张吉明,许庆方.Rep-PCR技术及其应用现状[J].中国养羊,2014(3):55-57. |
| |
| 作者姓名： | 赵燕梅 张吉明 许庆方 |
| |
| 作者单位： | 山西农业大学动物科技学院,太谷030801 |
| |
| 基金项目： | 公益性行业（农业）科研专项（201303061）;山西省科技攻关计划（20100311052）;“十二五”国家科技支撑计划（2011BAD17B02） |
| |
| 摘 要： | 文章着重介绍了Rep-PCR技术原理、特点及其在细菌、真菌、环境微生物遗传多样性研究方面的应用.Rep-PCR目前发展迅速并被广泛应用于多种细菌基因分类,此方法操作方便,可以大样本进行,且Rep-PCR分辨效果好,可重复性强.现在Rep-PCR可自动化分型,并可建立各种细菌REP、ERIC-PCR分型标准数据库.但Rep-PCR也存在一定问题,不同来源的或不同批次的引物和酶,对Rep-PCR结果都有一定的影响.
|
| 关 键 词： | Rep-PCR 细菌 真菌 环境微生物 遗传多样性 |
Application Status of Rep-PCR Technology |
| |
| Authors: | Zhao Yan-mei Zhang Ji-ming Xu Qing-fang |
| |
| Institution: | ( College of Animal Science and Technology, Shanxi Agricuhural University, Taigu 030801, China) |
| |
| Abstract: | This paper focuses on the principle,characteristics of Rep-PCR and its applications in bacteria,fungi,environmental microbial genetic diversity.Rep-PCR has been developed rapidly and applied widely in gene classification of bacteria,fungi,environmental microbial.The method can analyse large sample with conveniently.Besides,the Rep-PCR has a good resolution and repeatability.Currently,Rep-PCR is available to automatic classification,and establishing classification standard database of REP,ERIC-PCR.But Rep-PCR also has some problems,different sources or different batches of primers and enzymes have a certain influence on the result of Rep-PCR. |
| |
| Keywords: | Rep-PCR bacteria fungi environmental microbial genetic diversity |
| 本文献已被 维普 等数据库收录！ |
|
