| 用于RAPD分析的树莓基因组总DNA提取初报 |
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| 引用本文： | 卞贵建,路艳,周庆阳. 用于RAPD分析的树莓基因组总DNA提取初报[J]. 广东农业科学, 2007, 0(3): 36-38 |
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| 作者姓名： | 卞贵建 路艳 周庆阳 |
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| 作者单位： | 山东交通职业学院公路系,山东,潍坊,261206;潍坊学院生物工程学院,山东,潍坊,261061;四川农业大学林学园艺学院,四川,雅安,625014 |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | 以树莓嫩叶为材料,进行树莓基因组总DNA不同提取方法的比较试验.结果表明:CTAB法、SDS法、改良CTAB法均可成功提取树莓DNA,但无论在质量上还是在数量上,改良CTAB法都优于常规CTAB法和SDS法,所提取的DNA大多呈现无色透明状,A260/ A280值多数介于1.65～1.90,完全可以用于RAPD分析.
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| 关 键 词： | 树莓 RAPD分析 基因 DNA提取 |
| 文章编号： | 1004-874X(2007)03-0036-03 |
| 修稿时间： | 2006-12-06 |
Preliminary report of DNA extraction for RAPD an analysis in raspberry |
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| BIAN Gui-jian,LU Yan,ZHOU Qing-yang. Preliminary report of DNA extraction for RAPD an analysis in raspberry[J]. Guangdong Agricultural Sciences, 2007, 0(3): 36-38 |
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| Authors: | BIAN Gui-jian LU Yan ZHOU Qing-yang |
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| Abstract: | Total DNA was extracted from the raspberry young leaves and the different extraction methods were compared, which tried to find out the suitable method for the raspberry genomic total DNA extraction. The result indicated that the CTAB method, the SDS method, the improved CTAB method may successfully extract DNA, but regardless of the quality and the quantity, the improved CTAB method surpassed conventional CTAB, SDS, DNA extracted mostly presented the colorless transparency by the improved CTAB method, The A260/A280 values was between 1.65 - 1.90, This quality of DNA was suitable for RAPD analysis. |
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| Keywords: | raspberry RAPD analysis gene DNA extraction |
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