光周期对光敏核不育水稻小孢子发生和花粉发育的超微结构的影响

 以光敏核不育水稻农垦58S为材料，在杭州中国水稻研究所人工气候箱(Koitotron S-153W )群中进行光周期处理。自秧苗6叶期开始，先经10 h／d的短日照处理10 d 到幼穗开始分化，然后分别在13.14 h／d的光周期条件下发育。通过透射电镜观察探索光周期在决定花粉育性中的作用。结果发现，在13 h／d光周期条件，花粉发育正常，而在14h／d的光周期条件下，花粉在单核晚期败育，表现在核糖体呈聚合状态，内质网、质体、线粒体等细胞器逐渐解体，缺乏淀粉积累，吞噬泡增多，细胞质稀薄，最终变成不育。另一方面，绒毡层细胞保持完整状态，无解体迹象。

Ultrastructural Changes in Microsporogenesis and Pollen Development of Photoperiod Sensitive Genic Male-sterile Rice under Different Day Length

Hubei Photoperiod Sensitive Genic Male-sterile Rice Nongken 58S was treated with different photopcriods in phytotron chambers of Koitotron S-153W at China National Rice Research Institute, Hangzhou. The 6-leaf old seedlings were exposed to short photoperiod of 10 h/d for 10d, and differentiation of the panicle proceeded under photoperiods of 13 and 14 h/d respectively. Panicle primordia were fixed and sectioned for TEM examination to investigate the role of different photoperiods on pollen sterility. Under 13 h/d pollen formation was quite normal, but under 14 h/d most pollen grains aborted at the late uninucleate stage. The altrastructural abnormalities include: aggregation of the ribosomes: gradual disintegration of the endoplasmic reticula, plastids, mitochondria and other organelles; absence of starch accumulation: increase of phagocytic vacuoles; thinning of the cytoplasm etc. and eventually abortion of the pollen grains. On the other hand, the tapetum remained intact and no disintegration of tapetal cells happened.