The location of DNA in RecA-DNA helical filaments |
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| Authors: | E H Egelman X Yu |
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| Affiliation: | Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511. |
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| Abstract: | The helical filament that the RecA protein of Escherichia coli forms around DNA is the active apparatus in protein-catalyzed homologous genetic recombination. The actual position of DNA within this complex has been unknown. Image analysis has been performed on electron micrographs of filaments of RecA on double-stranded DNA and on single-stranded DNA to visualize a difference that is consistent with one strand of the double-stranded DNA. This localization of the DNA gives additional information about the unusual structure of DNA in the complex with RecA protein. |
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