不同猕猴桃品种RAPD分析及其与抗溃疡病的关系

对不同猕猴桃品种的分子生物学试验表明：猕猴桃的DNA浓度在920 μg/mL符合RAPD分析的要求。通过60个随机引物的PCR扩增，报道了6个不同品种和类型猕猴桃种质资源的RAPD多态性,计算了它们之间的遗传距离，构建了聚类图，并讨论了其亲缘关系。聚类分析图反映出来源于安徽省主要猕猴桃产区的6个样品可以分为3组，其中抗病与感病的相对较为集中，由此可推断出现这种聚类的原因可能是由于它们基因组中有相同的DNA片段。抗病品系都有一条1 458 bp DNA片段，而感病品系均没有该带。故该片段可能与猕猴桃植株抗溃疡病相关。RAPD多态性从分子水平上反映出了猕猴桃种质资源不同品种及不同类型间复杂的遗传背景，为抗病育种的亲本选配提供了依据，也为合成猕猴桃抗溃疡病探针并用于检测猕猴桃抗溃疡病种质和分子标记辅助育种奠定了基础。

Analysis of the relationships between different kiwifruit cultivars and their resistance to Pseudomonas syringae pv. Actinidiae by RAPD

RAPD (randomly amplified polymorphic DNA) was used to analyze the genomic DNAs of kiwifruit cultivars resistant to the bacterial canker with 60 decanucleotide arbitrary primers selected. The results revealed that 920 μg/mL of DNA from various kiwifruit cultivars was required for RAPD analysis. The genomic DNAs of 6 kiwifruit cultivars were amplified by RAPD with 60 arbitrary decamers. Their genetic distances were calculated, and the dendrogram was constructed by cluster analysis. The results showed that the 6 DNA samples from Anhui could be divided into 3 groups, of which the diseaseresistant and disease susceptible groups were placed in different groups. The data suggested that similar DNA fragments exist in the same group. An 1 458 bp DNA fragment exists in resistant cultivars, but no such fragment in susceptible cultivars. So the fragment was possibly in correlation with the resistance to bacterial canker in kiwifruit cultivars.