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| 象耳豆根结线虫果胶酸裂解酶基因的克隆及其RNAi 效应分析 | |
| 引用本文： | 卓侃,迟远丽,扈丽丽,罗梅,廖金铃. 象耳豆根结线虫果胶酸裂解酶基因的克隆及其RNAi 效应分析[J]. 植物病理学报, 2011, 41(5): 473-481 |
| 作者姓名： | 卓侃 迟远丽 扈丽丽 罗梅 廖金铃 |
| 作者单位： | 1. 华南农业大学资源环境学院植物线虫研究室,广州,510642 2. 华南农业大学资源环境学院植物线虫研究室,广州510642;珠海出入境检验检疫局,珠海519015 |
| 基金项目： | 国家自然科学基金，国家转基因生物新品种培育重大专项，国家公益性农业科研专项资助 |
| 摘 要： | 从象耳豆根结线虫中首次克隆了果胶酸裂解酶基因Me-pel-1,该基因cDNA 的开放阅读框(ORF)长813 bp,编码270 个氨基酸,具有果胶酸裂解酶第3 家族的4 个保守域特征。ME-PEL-1 与南方根结线虫的果胶酸裂解酶MI-PEL-1 和爪哇根结线虫的果胶酸裂解酶MJ-PEL-1 相似性最高,且系统进化树显示ME-PEL-1 也与它们最为接近。利用RNAi 技术对象耳豆根结线虫2 龄幼虫的Me-pel-1 进行沉默,结果发现Me-pel-1 基因被干扰后,象耳豆根结线虫2 龄幼虫对番茄的侵染率显著降低。该结果表明Me-pel-1 基因在象耳豆根结线虫侵染寄主的过程中发挥重要作用。 |
| 关 键 词： | 象耳豆根结线虫 果胶酸裂解酶 克隆 RNA 干扰 |
Cloning and RNA interference analysis of a pectate lyase gene of Meloidogyne enterolobii |
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| ZHUO Kan,CHI Yuan-li,HU Li-li,LUO Mei,LIAO Jin-ling. Cloning and RNA interference analysis of a pectate lyase gene of Meloidogyne enterolobii[J]. Acta Phytopathologica Sinica, 2011, 41(5): 473-481 | |
| Authors: | ZHUO Kan CHI Yuan-li HU Li-li LUO Mei LIAO Jin-ling |
| Affiliation: | ZHUO Kan1,CHI Yuan-li1,2,HU Li-li1,LUO Mei1,LIAO Jin-ling1(1Laboratory of Plant Nematology,College of Natural Resource and Environment,South China Agricultural University,Guangzhou 510642,China,2Zhuhai Entry-Exit Inspection and Quarantine Bureau,Zhuhai 519015,China) |
| Abstract: | Cloning and characterization of a cDNA encoding pectate lyase from the root-knot nematode Meloidogyne enterolobii is described firstly. The full length cDNA (Me-pel-1) containing a 813 bp open read-ing frame (ORF) that could be translated into a 270 amino acids polypeptide. The deduced protein including four conserved regions possesed characteristic of the class Ⅲ pectate lyases and showed the highest identity with MI-PEL-1 from M. incognita and MJ-PEL-1 from M. javanica. The phylogenetic tree based on the class Ⅲ pectate lyases also indicated ME-PEL-1 was the most closest to the two proteins mentioned above. Infection of tomato with secondary juveniles (J2s) of M. enterolobii incubated in dsRNA against Me-pel-1 resulted in lower infection efficiency, indicating the importance of the gene for plant penetration. |
| Keywords: | Meloidogyne enterolobii pectate lyase cloning RNAi |
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