氯化钠对分蘖葱头根尖细胞有丝分裂的影响及其致畸效应

以分蘖葱头根尖为试验材料,研究了氯化钠对分蘖葱头根尖的毒性效应。用不同浓度(0.05、0.1、0.2、0.3 mol/L)的氯化钠水溶液对分蘖葱头根尖细胞染毒12 h、24 h、48 h,观察分蘖葱头根尖细胞有丝分裂指数、微核率以及染色体畸变情况。结果表明:在试验剂量范围内,随着氯化钠水溶液浓度的升高,染毒时间增长,分蘖葱头根尖细胞的有丝分裂指数逐渐降低,染色体畸变率和微核率逐渐升高,并且均在氯化钠水溶液浓度为0.3 mol/L、染毒时间为48 h时达到最大值,说明氯化钠是一种胁迫因子。

Effect of Sodium Chloride on Mitotic Index and Chromosome Aberration of Root Tip Cell of Allium cepa var.aggreatum

With the root tip as experimental materials,toxic effect of sodium chloride on Allium cepa var.aggreatum root tip was investigated.The Allium cepa var.aggreatum root tip cells were exposed to aqueous solution of sodium chloride with different concentration(0.05,0.1,0.2,0.3 mol/L) for 12 h,24 h and 48 h,respectively.And then the mitotic index,micronucleus rate and chromosome aberration of the Allium cepa var.aggreatum root tip cell were investigated.The experimental results indicated that with the elevated concentrations of sodium chloride aqueous and the increase of exposing time,the mitotic index decreased,chromosome aberration rate and micronucleus rate increased gradually.Moreover,the maximum value was reached when the concentration of sodium chloride aqueous was 0.3 mol/L and the exposure time was 48 h,which indicated that sodium chloride is a kind of stress factor.