| UPLC法测定朝鲜蓟叶中绿原酸含量 |
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| 引用本文： | 张俊,杜刚,田孟华,苏海林,吕福聪,杨海英. UPLC法测定朝鲜蓟叶中绿原酸含量[J]. 北方园艺, 2011, 0(20): 46-48 |
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| 作者姓名： | 张俊 杜刚 田孟华 苏海林 吕福聪 杨海英 |
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| 作者单位： | 云南民族大学化学与生物技术学院,民族药资源化学重点实验室,云南昆明650031;云南民族大学化学与生物技术学院,民族药资源化学重点实验室,云南昆明650031;云南民族大学化学与生物技术学院,民族药资源化学重点实验室,云南昆明650031;云南民族大学化学与生物技术学院,民族药资源化学重点实验室,云南昆明650031;云南民族大学化学与生物技术学院,民族药资源化学重点实验室,云南昆明650031;云南民族大学化学与生物技术学院,民族药资源化学重点实验室,云南昆明650031 |
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| 基金项目： | 云南民族大学科研创新团队资助项目(201004),云南民族大学民族药资源化学国家民委-教育部重点实验室开放基金资助项目(MZY1108) |
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| 摘 要： | 采用Acquity BEH C18色谱柱(2.1 mm×100 mm,1.7 μm),乙腈-2％的乙酸水溶液(11∶89)等度洗脱,柱温:30℃,检测波长328 nm,流速:0.4 min/mL,进样量:1μL,研究了测定朝鲜蓟叶中绿原酸含量的超高效液相色谱方法.结果表明:绿原酸在0.013～1.3 mg/mL范围类线性关系良好,平均回收率为99.10％,RSD为1.87％,与常规HPLC方法比较,该方法分析时间短,节约溶剂,适用于朝鲜蓟叶中绿原酸含量的快速测定.
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| 关 键 词： | 超高效液相 朝鲜蓟 绿原酸 |
Determination of Chlorogenic Acid in Leaves of Cynara scolymus L.by UPLC |
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| ZHANG Jun , DU Gang , TIAN Meng-hua , SU Hai-lin , LV Fu-cong , YANG Hai-ying. Determination of Chlorogenic Acid in Leaves of Cynara scolymus L.by UPLC[J]. Northern Horticulture, 2011, 0(20): 46-48 |
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| Authors: | ZHANG Jun DU Gang TIAN Meng-hua SU Hai-lin LV Fu-cong YANG Hai-ying |
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| Affiliation: | ZHANG Jun,DU Gang,TIAN Meng-hua,SU Hai-lin,LV Fu-cong,YANG Hai-ying(Key Laboratory of Nationalities Medicine Kesources,School of Chemistry and Biotechnology,Yunnan University of Nationalities,Kunming,Yunnan 650031) |
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| Abstract: | The sample was extracted with water using regurgitation,UPLC separation was carried out in a Acquity BEH C18 column(2.1 mm×100 mm,1.7 μm)with acetomitrile-water-aceticacid(11∶87∶2)as mobile phase.The detection wave length was 328 nm,the Velocity was 0.4 min/mL,the column temperature was 30℃,incoming sample amount 1 μL.To develop an UPLC method for the determination of chlorogenic acid in leaves of Cynara scolymus L.The results showed that linear range was 0.013~1.3 mg/mL for chlorogenic acid.The average recovery was 99.10%,and RSD was 1.87%.Compared with conventional method HPLC,this established method could save analysis of time and solvent for determination of chlorogenic acid in leaves of Cynara scolymus L. |
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| Keywords: | UPLC Cynara scolymus L. chlorogenic acid |
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