| 苹果体细胞悬浮培养及植株再生研究 |
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| 引用本文： | 李玉生,吴永杰,赵艳华,吴雅琴,程和禾,陈龙.苹果体细胞悬浮培养及植株再生研究[J].北方园艺,2011(19):103-106. |
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| 作者姓名： | 李玉生 吴永杰 赵艳华 吴雅琴 程和禾 陈龙 |
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| 作者单位： | 河北省农林科学院昌黎果树研究所,河北昌黎,066600;河北省农林科学院昌黎果树研究所,河北昌黎,066600;河北省农林科学院昌黎果树研究所,河北昌黎,066600;河北省农林科学院昌黎果树研究所,河北昌黎,066600;河北省农林科学院昌黎果树研究所,河北昌黎,066600;河北省农林科学院昌黎果树研究所,河北昌黎,066600 |
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| 基金项目： | 河北省自然科学基金资助项目(C2007000968) |
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| 摘 要： | 以苹果试管苗叶片的再生不定芽嫩叶为试材,对苹果体细胞悬浮系的建立及植株再生的影响因素进行了研究.结果表明:在MS+NAA 0.5 mg/L+BA 2.0 mg/L培养基上可诱导获得高活力的愈伤组织.将该愈伤组织转入MS+2,4-D 2.0 mg/L+BA 1.0 mg/L液体培养基中培养,采用无菌筛网分离获得含单个细胞和少于8～10个细胞的小细胞团进行继代培养,建立体悬浮细胞培养系.将悬浮细胞转到MS+2,4-D 2.0 mg/L+BA 1.0 mg/L的固体增殖培养基上暗培养25 d后,可形成微型愈伤组织,将该愈伤组织转到MS+BA 5.0 mg/L+IAA 0.5 mg/L的植株再生培养基上暗培养30 d后转入光下,光照培养30 d后53％的愈伤组织可再生植株.该研究建立的苹果体细胞悬浮培养技术,不仅可用于细胞融合及遗传转化过程中杂种细胞和转化细胞的分离及植株再生研究,而且对于利用组织培养技术筛选变异株系也具有重要意义.
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| 关 键 词： | 苹果 愈伤组织 悬浮培养 植株再生 |
Study on the Somatic Cell Suspension Culture and Plant Regeneration of Apple |
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| LI Yu-sheng
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WU Yong-jie
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ZHAO Yan-hua
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WU Ya-qin
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CHENG He-he
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CHEN Long.Study on the Somatic Cell Suspension Culture and Plant Regeneration of Apple[J].Northern Horticulture,2011(19):103-106. |
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| Authors: | LI Yu-sheng WU Yong-jie ZHAO Yan-hua WU Ya-qin CHENG He-he CHEN Long |
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| Institution: | LI Yu-sheng,WU Yong-jie,ZHAO Yan-hua,WU Ya-qin,CHENG He-he,CHEN Long(Changli Institute of Pomology,Hebei Academy of Agriculture and Forestry Sciences,Changli,Hebei 066600) |
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| Abstract: | The young leaves of plantlets regenerated from the leaves of apple seedling in vitro were used test material,effect factors of the establishment of suspension cultures from apple somatic cell and plant regeneration were studied.The results indicated that callus with strong growth vitality could be induced that cultured on MS medium supplemented with 0.5 mg/L NAA,and 2.0 mg/L BA.The callus were transferred to liquid MS medium supplemented with 2.0 mg/L 2,4-D,1.0 mg/L BA and the suspension cell lines were est... |
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| Keywords: | apple callus suspension culture plant regeneration |
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