| 白菜软腐病拮抗菌M-14拮抗机理和拮抗物质的初步研究 |
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| 引用本文： | 唐蕊,张雪辉,石晓云,王僧虎.白菜软腐病拮抗菌M-14拮抗机理和拮抗物质的初步研究[J].北方园艺,2011(20):38-39. |
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| 作者姓名： | 唐蕊 张雪辉 石晓云 王僧虎 |
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| 作者单位： | 邢台学院生物化学系,河北邢台,054001;邢台学院生物化学系,河北邢台,054001;邢台学院生物化学系,河北邢台,054001;邢台学院生物化学系,河北邢台,054001 |
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| 基金项目： | 河北省科技支撑计划资助项目(10220307) |
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| 摘 要： | 通过室内筛选得到对白菜软腐病有较强拮抗作用的菌株M-14,对其拮抗机理、拮抗物质的稳定性及抗菌谱进行了初步研究.结果表明:拮抗菌M-14对白菜软腐病菌的拮抗作用源于代谢物质而不是菌体本身；M-14酸碱稳定性极强,在pH 3～11的范围内均表现出较强的抑茵效果；拮抗菌M-14产生的拮抗物质热稳定性较强,在40℃时抑菌活性最强,10～100℃范围内均表现较强的抑茵活性；经对5种供试菌的抑菌作用测试,表现出抗菌谱广的特点.
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| 关 键 词： | 白菜软腐病 拮抗菌 拮抗物质 拮抗机理 |
Primary Study on the Antibiotic Mechanism and Substance of M-14 Strain Against Chinese Cabbage Soft Rot |
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| TANG Rui
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ZHANG Xue-hui
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SHI Xiao-yun
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WANG Seng-hu.Primary Study on the Antibiotic Mechanism and Substance of M-14 Strain Against Chinese Cabbage Soft Rot[J].Northern Horticulture,2011(20):38-39. |
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| Authors: | TANG Rui ZHANG Xue-hui SHI Xiao-yun WANG Seng-hu |
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| Institution: | TANG Rui,ZHANG Xue-hui,SHI Xiao-yun,WANG Seng-hu(Department of Biology and Chemistry,Xingtai University,Xingtai,Hebei 054001) |
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| Abstract: | M-14 strain was selected by screening against Chinese cabbage soft rot,the antibiotic mechanism,substance and antimicrobial spectrum were studied.The results showed that the antagonism of M-14 to Chinese cabbage soft rot from metabolites rather than itself bacteria,antibiotic activity was due to the producing substance of M-14 strain.it was very stable in 3~11 pH values and 10~100℃ temperatures.In addition,it could inhibit 3 bacteria and 2 fungi,so its restraint spectrum was very wide. |
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| Keywords: | Erwinia carotovora antagonistic bacteria antibiotic mechanism and substance antibiotic mechanism |
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