| 黄瓜矮化突变体再生体系的建立 |
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| 引用本文： | 黄莎莎,王多佳,李凤兰,胡宝忠. 黄瓜矮化突变体再生体系的建立[J]. 北方园艺, 2011, 0(19): 110-112 |
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| 作者姓名： | 黄莎莎 王多佳 李凤兰 胡宝忠 |
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| 作者单位： | 东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030 |
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| 基金项目： | 黑龙江省自然科学基金资助项目(230340); 高等学校博士点专项科研基金资助项目(200802240008) |
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| 摘 要： | 以黄瓜矮化突变体D0462子叶为外植体,以MS为基本培养基,建立黄瓜矮化突变体再生体系.结果表明:不定芽诱导最适培养基为MS+1.0 mg/L 6-BA;芽伸长最适培养基为MS+0.1 mg/L 6-BA;再生植株生根培养基为1/2MS.为了控制芽伸长阶段出现的开花现象,选用6-BA浓度0.1 mg/L,光照时间8 h/d,尽可能抑制开花.
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| 关 键 词： | 黄瓜 矮化突变体 再生体系 不定芽 光照 |
Construction of Dwarf Cucumber Regeneration System |
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| HUANG Sha-sha , WANG Duo-ji , LI Feng-lan , HU Bao-zhong. Construction of Dwarf Cucumber Regeneration System[J]. Northern Horticulture, 2011, 0(19): 110-112 |
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| Authors: | HUANG Sha-sha WANG Duo-ji LI Feng-lan HU Bao-zhong |
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| Affiliation: | HUANG Sha-sha,WANG Duo-jia,LI Feng-lan,HU Bao-zhong(College of Life Science,Northeast Agricultural University,Harbin,Heilongjiang 150030) |
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| Abstract: | Taking cotyledon of dwarf cucumber 'D0462'as explants,using MS medium as the basic medium,regeneration system of dwarf cucumber mutant were established.The results showed that the best medium for inducted adventitious bud was MS+1.0 mg/L 6-BA;optimization medium for shoots elongation were MS+0.1 mg/L 6-BA;roots regeneration medium was 1/2MS;In order to control the flowering during bud elongation,by alter 6-BA hormone concentration 0.1 mg/L and illumination time 8 h/d,inhibition explode as much as possible. |
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| Keywords: | cucumber dwarfed mutant regeneration system adventitious shoot illumination |
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