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常见观赏竹类过氧化物酶同工酶及可溶性蛋白分析
引用本文:孙婴宁,王维禹,方姝. 常见观赏竹类过氧化物酶同工酶及可溶性蛋白分析[J]. 北方园艺, 2011, 0(21): 110-112
作者姓名:孙婴宁  王维禹  方姝
作者单位:齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔,161006;齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔,161006;齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔,161006
摘    要:为了探讨常见观赏竹类的亲缘关系,采用聚丙烯酰胺凝胶电泳(PAGE)技术分析了竹类植物过氧化物酶同工酶和可溶性蛋白.结果表明:供试材料的过氧化物酶同工酶和可溶性蛋白的位点和数目均存在差异.相似性系数在0.400~0.762,同一种属的金边富贵竹与青叶富贵竹相似性系数最高(0.762).聚类结果显示,6个供试样品聚成二大类,与形态分类结果基本一致.试验结果可以初步作出常见观赏竹类品种间亲缘关系的判定.

关 键 词:观赏竹  同工酶  过氧化物酶(POD)  可溶性蛋白

Analysis Peroxidase Isozyme and Soluble Protein in Ornamental Bamboos
SUN Ying-ning , WANG Wei-yu , FANG Shu. Analysis Peroxidase Isozyme and Soluble Protein in Ornamental Bamboos[J]. Northern Horticulture, 2011, 0(21): 110-112
Authors:SUN Ying-ning    WANG Wei-yu    FANG Shu
Affiliation:SUN Ying-ning,WANG Wei-yu,FANG Shu(College of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihar,Heilongjiang 161006)
Abstract:In order to research the genetic differences of ornamental bamboos,the peroxidase isozyme of bamboo leaves were analyzed by plumb plate of polyarclamide gel electrophoresis.The results showed that there were certain differences in the number and locus of the peroxidase isozyme and soluble protein bands among the accessions.The similarity coefficient was 0.400~0.762.The similarity coefficient between the two varieties of Dracaena sanderiana was the highest(0.762).The clustering analysis indicated that all sa...
Keywords:ornamental bamboo  isozyme  peroxidase  soluble protein  
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