Bovine alveolar macrophage neurokinin-1 and response to substance P |
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Authors: | Rogers Donna P Wyatt Carol R Walz Paul H Drouillard James S Mosier Derek A |
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Affiliation: | Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, USA. |
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Abstract: | In this study bovine alveolar macrophage neurokinin-1 (NK-1) and the in vitro response to substance P (SP) exposure were investigated. Bovine alveolar macrophage membrane extracts separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted using anti-NK-1 antiserum demonstrated the presence of an approximately 60kDa band. Phagocytosis of fluorescent bioparticles by SP-exposed macrophages was 39% greater than that of non-exposed macrophages (P=0.0089). Likewise, there was 28% greater TNF production by macrophages following SP exposure compared to non-exposed controls (P=0.116). These results suggest that bovine alveolar macrophages respond to SP at least in part by enhancing phagocytosis and TNF production. |
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