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| 小金海棠超氧化物歧化酶基因MxSod2克隆与序列分析 | |
| 引用本文： | 张玉刚,郭绍霞,韩振海.小金海棠超氧化物歧化酶基因MxSod2克隆与序列分析[J].中国农学通报,2009,25(4):43-46. |
| 作者姓名： | 张玉刚 郭绍霞 韩振海 |
| 作者单位： | 1. 青岛农业大学园林园艺学院,山东青岛,266109;中国农业大学园艺植物研究所,北京,100193 2. 青岛农业大学园林园艺学院,山东青岛,266109 3. 中国农业大学园艺植物研究所,北京,100193 |
| 基金项目： | 北京市自然科学基金重点项目 |
| 摘 要： | 本实验以苹果属植物小金海棠（Malus xiaojinensis Cheng et Jiang）为试材，利用差异筛选消减cDNA文库和RACE相结合的方法，分离到了小金海棠超氧化物歧化酶基因MxSod2（Genbank登录号为 DQ431473），该基因全长805个碱基，开放阅读框为471bp，编码157个氨基酸，推测分子量15kDa。该基因具有CuZn-SOD基因的典型结构域特征。通过系统进化树分析，小金海棠MxSod2与龙眼、荔枝等非禾本科植物保持了较近的亲缘关系，与花生、水稻、玉米等禾本科植物亲缘关系较远。 |
| 关 键 词： | 石油污染 石油污染 土壤修复 植物筛选 |
| 收稿时间： | 2008-10-30 |
| 修稿时间： | 2008-12-18 |
Cloning and Sequence Analysis of Superoxide dismutase gene (MxSod2) in Malus xiaojinensis |
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| Zhang Yu-gang,Guo Shao-xia,Han Zhen-hai.Cloning and Sequence Analysis of Superoxide dismutase gene (MxSod2) in Malus xiaojinensis[J].Chinese Agricultural Science Bulletin,2009,25(4):43-46. | |
| Authors: | Zhang Yu-gang Guo Shao-xia Han Zhen-hai |
| Institution: | Zhang Yugang ;Guo Shaoxia ;Han Zhenhai (1 College of Horticulture;Qingdao Agricultural University;Qingdao Shandong 266109;2 Institute for Horticultural Plants;China Agricultural University;Beijing 100193) |
| Abstract: | A superoxide dismutase gene was cloned by differential screening of the subtracted cDNA library and RACE from Malus xiaojinensis Cheng et Jiang, which was named MxSod2 (Accession No. DQ431473). The full-length cDNA of MxSod2 is 805 bp, including the open reading frame (ORF) of 471 bp. It encodes a protein of 157 amino acids, with a molecular weight of 15 kDa. The MxSod2 contained the conserved domains shared in all known SOD. The phylogenetic tree suggested that MxSod2 was closer to the SOD of non-graminaceous plants including longan and litchi, but farther with the SOD of peanut, rice and maize. |
| Keywords: | MxSod2 |
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