银杏非根尖细胞的染色体观察

由于雌雄异株植物银杏的种子及幼苗无法区分性别 ,因而在研究其性别时不能用传统的种子萌发取根的方法来进行染色体制片 ,而从雌、雄成株上取根也不切实可行。为解决该问题 ,本文以银杏的幼小花药、叶、胚珠以及小孢子、雌配子体游离核为材料 ,主要采用放线菌酮 (Cycloheximide,1 0 0mg/kg)作预处理药物 ,常规制片均得到了优良的染色体标本。

OBSERVATION ON CHROMOSOMES OF GINKGO BILOBA BY USING MATERIALS RATHER THAN ROOT-TIP CELLS

The sex of anyone of seeds and seedlings of dioecious \%Ginkgo biloba \%can not be identified, so we are unable to use their roots as materials to make chromosomes samples according to the traditional method while we study the sex of \%Ginkgo biloba.\% Moreover, it isn't feasible to collect roots from mature pistillate and staminate plants. In order to resolve this problem, we used young anthers, leaves, ovules, microspores and female gametophyte in free\|nuclear stage of \%Ginkgo biloba \%respectively as plant materials and used cycloheximide (100 mg/kg) to treat plant materials alive. We have obtained wonderful chromosomes samples in the end. Pretreatment with cycloheximide is also discussed briefly.