植物逆境诱导启动子mwcs120的克隆及表达特性研究

以小麦基因组DNA为模板，通过特异PCR扩增，克隆逆境诱导表达启动子mwcs120。序列分析表明，该启动子与冷诱导启动子wcs120的序列同源性为97.1%，有2个位点发生了突变，但逆境调控保守元件的序列未发生改变。瞬时表达实验表明，在单子叶和双子叶植物中，mwcs120启动子均受低温和高盐逆境诱导，使GUS基因的表达增强。因此，mwcs120启动子在作物抗逆基因工程中具有较好的应用前景。

Cloning and Expression Properties of Plant Stress Inducible Promoter mwcs120

Growth and development of plant are heavily influenced by abiotic stress.In recent years,different resistant genes have been cloned and transferred into less resistant species.It was attempted to improve crops for their resistance to abiotic stress.In most cases,however,forceful constitutive promoter was used to promote over expression of stress gene.This was disadvantageous to some activities of physiological metabolism,although the transgenic plant was improved for its stress resistance.In this study,stress inducible promoter mwcs120 was cloned by specific PCR from wheat genomic DNA.Sequence analysis showed that this promoter was 97.1% homologous with cold inducible promoter wcs120.Even though mutations were found at two locations,conservative elements for stress regulation were still kept unchanged.Transient expression experiment indicated that,both in monocotyledonous and dicotyledonous plants,promoter mwcs120 was induced by low temperature and high salt stress to increase expression of gene GUS.Therefore,promoter mwcs120 was hopeful to be used to gene engineering of crop improvement for stress resistance.