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一种茶枝致病菌的分离鉴定及致病力分析
引用本文:张兰,郭华伟,李梦菡,廖杨文科,李鑫,韩文炎. 一种茶枝致病菌的分离鉴定及致病力分析[J]. 茶叶科学, 2019, 39(6): 661-668. DOI: 10.13305/j.cnki.jts.2019.06.005
作者姓名:张兰  郭华伟  李梦菡  廖杨文科  李鑫  韩文炎
作者单位:中国农业科学院茶叶研究所,浙江 杭州,310008;中国农业科学院茶叶研究所,浙江 杭州 310008;中国农业科学院研究生院,北京 100081;南京林业大学生物与环境学院南方现代林业协同创新中心,江苏 南京,210037
基金项目:国家重点研发计划政府间国际科技创新合作重点专项(2017YFE0107500)、浙江省自然科学基金(LY19C160009)、中国农业科学院科技创新工程、国家自然科学基金(31600482)
摘    要:采用形态学和分子鉴定方法确定采自建德市大同镇茶园茶枝的致病菌为镰刀菌Fusarium incarnatum与茶炭疽菌Colletotrichum fructicola,鉴定并报道F. incarnatum为茶树致病菌。龙井43和中茶108离体叶片及茎秆用于两种病原菌致病力分析试验,结果显示,F. incarnatum对龙井43嫩叶及茎秆具有较强侵染力,是造成该茶枝茎秆病害的主要致病菌,并且可能与C. fructicola共同侵染茶树叶片。因此,在今后茶树病害识别与防控方面需要密切关注。

关 键 词:形态学鉴定  分子鉴定  茶枝  镰刀菌  致病力
收稿时间:2019-06-11

Identification and Pathogenicity Analysis of Pathogens in Tea Branches
ZHANG Lan,GUO Huawei,LI Menghan,LIAO-YANG Wenke,LI Xin,HAN Wenyan. Identification and Pathogenicity Analysis of Pathogens in Tea Branches[J]. Journal of Tea Science, 2019, 39(6): 661-668. DOI: 10.13305/j.cnki.jts.2019.06.005
Authors:ZHANG Lan  GUO Huawei  LI Menghan  LIAO-YANG Wenke  LI Xin  HAN Wenyan
Affiliation:1. Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China; 2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3. Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
Abstract:The pathogens of diseased tea branches in tea garden of Datong town, Jiande city were identified by morphological and molecular methods. Two fungal pathogens were identified, namely Fusarium incarnatum and Colletotrichum fructicola. F. incarnatum here was reported as the pathogen of tea trees. Leaves and stems of Longjing43 and Zhongcha108 were used in the experiment to analyze the pathogenicity of the two pathogens. The results show that F. incarnatum had strong pathogenicity to young leaves and stems of Longjing43, indicating it is the main pathogen causing relative disease. F. incarnatum and C. fructicola might infect tea leaves together which needs more attention in tea disease recognition, control and prevention in future.
Keywords:morphological identification  molecular identification  tea branch  Fusarium  pathogenicity  
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