凤仙花花叶病的病原鉴定

<正>0引言凤仙花(Impatiens balsamina)为凤仙花科(Balsaminaceae)凤仙花属(Impatiens)一年生草本植物，在我国大部分地区均有分布。栽培过程中凤仙花真菌病害主要有白粉病、褐斑病、炭疽病和立枯病等^1-2]。有关凤仙花病毒病的报道，如番茄斑萎病毒(tomato spotted wilt virus,TSWV)^3]、凤仙花坏死斑点病毒(impatiens necrotic spot virus,INSV)^4]、黄瓜花叶病毒(cucumber mosaic virus,CMV)^5-6]、烟草花叶病毒(tobacco mosaic virus,TMV)^7]、番茄花叶病毒(tomato mosaic virus,ToMV)^8]、长叶车前花叶病毒(ribgrass mosaic virus,RMV)^9]等均可侵染凤仙花。

Identification of the pathogen causing Impatiens balsamina mosaic disease

In 2018, some Impatiens balsamina plants exhibiting viral symptoms like chlorosis and mosaic were observed in the herb garden of Changchun University of Chinese Medicine in Changchun city, Jilin province. Systemic mosaic symptoms of Chenopodium amaranticolor and Nicotiana glutinosa mechanically inoculated with the sap of the diseased I. balsamina leaves were found. To further identify the pathogen of the diseased I. balsamina, high-throughput siRNA sequencing was performed and further verified by RT-PCR. The results showed that the diseased I. balsamina plants were mainly infected by alfalfa mosaic virus (AMV). The sequences of p1, p2, mp and cp genes of AMV I. balsamina (AMV-Ib) isolate were obtained by RT-PCR. Phylogenetic analysis based on the nucleotide sequence of the coat protein genes of AMV-Ib isolate showed that it is closely related to the subgroup I of AMV isolates. To our knowledge, this is the world’s first report of natural AMV infection on I. balsamina.