河南温县地黄上瓜类褪绿黄化病毒的鉴定及其CP序列分析

<正>0引言地黄(Rehmannia glutinosa Libosch．)为玄参科(Scrophulariaceae)多年生草本植物，根部为传统中药材，主要产区分布在山东、山西、河南、河北、北京等地。地黄栽培中通常使用块根进行无性繁殖，易导致病毒病积累发生，从而严重影响地黄的产量与质量^1]。目前侵染地黄的植物病毒主要有烟草花叶病毒(tobacco mosaic virus,TMV)、地黄花叶病毒(rehmannia mosaic virus,ReMV)、油菜花叶病毒(youcai mosaic virus,YoMV)、蚕豆萎蔫病毒2号(broad bean wilt virus 2,BBWV2)、车前草花叶病毒(plantago asiatica mosaic virus,P1AMV)等^2]。

Identification and CP sequences analysis of cucurbit chlorotic yellows virus from Rehmannia glutinosa in Wenxian County,Henan Province

In August of 2019, the leaves of Rehmannia glutinosa exhibiting chlorotic patches, mosaic, and irregular yellowing symptoms were observed in Chinese planting area located in WenXian Country of Henan Province. Total RNA was extracted from R. glutinosa samples that were suspected to be infected by viruses, and the siRNAs were obtained. After siRNA library construction, high-throughput sequencing was performed. The obtained reads were assembled by Velvet Software and analyzed by BLASTn in NCBI. The deep sequencing results showed that these samples were mainly infected with cucurbit chlorotic yellows virus (CCYV). Furthermore, RT-PCR and ELISA analyses showed that CCYV could naturally infect R. glutinosa. The coat protein (CP) coding sequence of CCYV R. glutinosa isolate was cloned and sequenced. The genetic variation was analyzed by constructing the phylogenetic tree based on the CP gene sequences. This study enriched the genetic information of CCYV population and provided a basis for the monitoring and further research on CCYV infecting R. glutinosa.