猕猴桃ACC合成酶基因家族四个成员的克隆 |
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引用本文: | 徐昌杰,陈昆松,张上隆.猕猴桃ACC合成酶基因家族四个成员的克隆[J].农业生物技术学报,2001,9(1):55-57. |
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作者姓名: | 徐昌杰 陈昆松 张上隆 |
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作者单位: | 1. 浙江大学农业与生物技术学院园艺系, 2. 浙江大学农业与生物技术学院园艺系;浙江大学生命科学院生物科学系 |
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基金项目: | 国家重点基础研究发展规划项目(973项目)(G2000046806),国家自然科学基金和浙江省自然科学基金资助 |
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摘 要: | 通过PCR方法从中华猕猴桃中分离出ACC合成酶基因家族的四个成员(AC-ACS1A、AC-ACS1B、AC-ACS2和AC-ACS3)的基因组DNA片段,AC-ACS1A、AC-ACS1B和AC-ACS2与其它植物该基因的氨基酸序列同源性最高可达76%以上,而AC-ACS3与其它植物ACC合成酶基因的氨基酸序列同源性均低于60%,与已知的其它猕猴桃ACC合成酶基因的同源性在51%-56%之间,且不存在MSSFGL保守区,因而属于一个未见报道的新成员。
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关 键 词: | 中华猕猴桃 ACC合成酶基因 基因家族 基因克隆 |
修稿时间: | 2000年7月3日 |
Molecular Cloning of Four
Members of ACC Synthase Gene Family fromKiwifruit(Actinidia chinensis Planch.) |
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Abstract: | Four members of 1-aminocyclopropane-1-carboxylate synthase(ACC synthase)gene family was isolated from Actinidia chinensis with the
assigned names:AC-ACS1A,AC-ACS1B,AC-ACS2 and AC-ACS3 by PCR.The amino acid sequence of AC-ACS1A,AC-ACS1B and AC-ACS2 are over 76% identical to some ACC synthase from other plants,while AC-ACS3 shows only 51%~56% nucleotide or amino acid sequence homology to other known kiwifruit ACC synthase genes,and its amino acid sequence is less than 60% identical to all known plant ACC synthases.AC-ACS3 fragment is a little shorter than other kiwifruit ACC synthase genes,and does not contain MSSFGL conserved region.Therefore,it is suggested that AC-ACS3 is a novel member of ACC synthase gene family. |
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