In Vitro Study of Adherence,Invasion, and Persistence of Streptococcus iniae in Fibroblastic-Like Fish Cell Line SAF-1 |
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| Authors: | F El Aamri F Real F Acosta B Acosta J Valdivia J Ramos-Vivas |
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| Institution: | 1. University Institute of Animal Health (IUSA) , University of Las Palmas de Gran Canaria , 35413 Arucas, Las Palmas , Spain;2. Department of Microbiology and Department of Immunology, Santa Cruz de Liencres Hospital , Marqués de Valdecilla Foundation , 39120 Santander, Spain |
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| Abstract: | Abstract Streptococcus iniae is a major fish pathogen producing invasive infections that result in economic losses in aquaculture. Gentamicin protection assays were used to investigate the ability of different S. iniae strains to invade and adhere to fibroblastic-like fish cell line SAF-1. All strains tested were detected intracellularly using both techniques, with variable internalization degrees between strains. The experiments carried out at 4°C demonstrated that active cell metabolism is necessary for bacterial internalization. Intracellular bacteria were detected for up to 3 d with a round morphology and were stained with 4′,6-diamidino-2-phenylindole (DAPI), indicating that some bacterial cells may remain viable inside SAF-1 cells. Our in vitro findings indicate that S. iniae is capable of adhering, entering, and surviving within fibroblastic cells, which may be important for the persistence and establishment of a carrier state. Received July 8, 2011; accepted February 12, 2012 |
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