Comparison of milk protein composition in a Swedish and a Danish dairy herd using reversed phase HPLC |
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Authors: | Anna Wedholm Elin Hallén Lotte Bach Larsen Helena Lindmark-Månsson Anders Hans Karlsson Toomas Allmere |
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Affiliation: | 1. Department of Food Science , Swedish University of Agricultural Sciences , Uppsala, Sweden anna.wedholm@lmv.slu.se;3. Department of Food Science , Swedish University of Agricultural Sciences , Uppsala, Sweden;4. Department of Food Science , Danish Institute of Agricultural Sciences, Research Centre Foulum , Tjele, Denmark;5. Swedish Dairy Association , Lund, Sweden;6. Department of Food Science , The Royal Veterinary and Agricultural University (KVL) , Fredriksberg C, Denmark |
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Abstract: | Abstract The milk composition in a Swedish herd, consisting of Swedish Red and White cows (SRB) and Swedish Holstein cows (SLB), and in a Danish herd, consisting of Danish Holstein-Friesian cows (SDM), was evaluated. Concentrations of the major milk proteins (κ-casein, αS1-casein, β-casein, β-lactoglobulin A, β-lactoglobulin B and α-lactalbumin) of 134 individual milk samples were determined by reversed phase (RP) HPLC. Other parameters determined included milk fat, urea, lactose, calcium, lactoferrin, somatic cell count and protein degradation (determined as level of free amino-terminals). Analysis of variance was used to compare concentrations of analysed milk variables between SRB and SLB or between SLB and SDM. Concentration of total protein, total casein, β-casein and κ-casein were significantly higher in SRB milk compared with SLB milk. Concentration of α-lactalbumin and calcium were significantly higher in SDM milk than in SLB milk. The concentration of urea was higher in SLB than in SDM milk and is suggested to reflect differences in feeding regimes between the investigated Swedish and Danish herd. |
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Keywords: | Calcium casein dairy cows free amino terminals urea whey protein |
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