Genotyping of Infectious Pancreatic Necrosis Virus Isolates from Mexico State |
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| Authors: | Magda Barrera-Mejía Simón Martínez César Ortega Raúl Ulloa-Arvizu |
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| Institution: | 1. Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Carretera de Cuota Toluca-Atlacomulco, Kilómetro 15.5, Código Postal 50200 , Toluca , Estado de México;2. Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia , Universidad Nacional Autónoma de México, Ciudad Universitaria D.F. , 04510 , México |
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| Abstract: | Abstract The infectious pancreatic necrosis virus (IPNV; genus Aquabirnavirus) affects salmon and trout, causing high mortality in first-feeding fry. The classification of this virus includes nine serotypes and seven genogroups. In Mexico, two different isolates were identified in 2000 and 2008, respectively. Both isolates were classified into genogroup I according to the RNA genome of this virus. As Mexico is importing rainbow trout Oncorhynchus mykiss eggs from different countries, the aim of this study was to genotype IPNV isolates obtained from four rainbow trout producer regions within the state of Mexico. We utilized a fragment of the VP2* (outer capsid protein) gene sequence of Mexican IPNV isolates as a molecular marker to determine the genogroup to which they belong. Although all Mexican IPNV isolates were grouped into genogroup I, we identified genetic diversity among these isolates, and 14 unique nucleotide sequence types were associated with the four producer regions in Mexico State. Received December 21, 2010; accepted July 27, 2011 |
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